Mar 28, · This protocol describes how to isolate primary cardiomyocytes from adult zebrafish hearts and culture them for up to 4 weeks, thereby using them as an alternative to in vivo experiments. After Cited by: Here we provide important information regarding the most prominent factors to attend to during adult cardiomyocyte isolation. At this point, the Langendorff portion of the isolation protocol is complete as there is no longer adequate perfusion through the coronary arteries. The end-point of the isolation procedure in a constant flow.
While such studies require a high quality cardiomyocyte population, successful cell isolation and maintenance during culture remain challenging. In this review, we describe methods for the isolation of adult and neonatal ventricular myocytes from rat and mouse heart. This protocol describes the isolation of cardiomyocytes from an adult mouse heart in preparation for dispensing and analysis on the ICELL8 system. The isolation protocol is mainly adapted from the published work by O'Connell, Rodrigo, and Simpson in
Adult mouse myocyte harvest protocol Protocol for adult mouse myocyte isolation edited from a protocol kindly provided by: Dr. Richard Pattern Tufts-New England Medical Centre Molecular Cardiology Research Centre Boston, MA Procedure: 1. Prepare the following solutions. The base solution is Hanks Balanced Salt Solution. This manuscript presents a protocol for successful isolation and cultivation of adult rat ventricular cardiomyocytes (ARVC). The rat is sacrificed by cervical dislocation under deep anesthesia. Then, the heart is extracted and the aorta is penetrat.xyz by:
We have developed a straightforward, reproducible protocol for isolating and culturing large numbers of adult mouse cardiac myocytes. This protocol is based on the traditional approach of retrograde perfusion of collagenase through the coronary arteries to digest the extracellular matrix of the heart and release rod-shaped penetrat.xyz by: Sep 18, · Isolation of high-quantity and high-quality ventricular cardiomyocytes from adult rats is critical to study heart physiology and pathology and for drug toxicity screening. It remains challenging to produce a high yield of viable cardiomyocytes from penetrat.xyz: Xiangang Tian, Meng Gao, Anqi Li, Bilin Liu, Wenting Jiang, Yuan Qin, Guohua Gong.